The 25??L PCR reaction volumes had been 50 KCl that is mM 10 mM Tris?HCl...
The 25??L PCR reaction volumes had been 50 mM KCl, 10 mM Tris?HCl, 2.5 mM MgCl2, 0.2 mM each dNTP, 0.2 ?M forward primer, 0.2 ?M reverse primer, 0.05 units/?L LGC Biotecnologia Taq DNA Polymerase, and included about 5–10 ng of genomic DNA. PCR conditions had been the following: denaturation at 93°C for 35 s, primer annealing at 50°C (cytochrome b ) or 55°C (control area and SRY/SRX) for 35 s, and extension that is primer 72°C for 90 s; these three actions had been duplicated 35 times.Read more
